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- KD/KO Validated
S100A6 Polyclonal antibody
S100A6 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells |
Positive IP detected in | A549 cells |
Positive IHC detected in | human stomach cancer tissue, human liver cancer tissue, human pancreas cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10245-1-AP targets S100A6 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, monkey |
Immunogen | S100A6 fusion protein Ag0385 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | S100 calcium binding protein A6 |
Synonyms | 2A9, 5B10, CABP, CACY, Calcyclin, MLN 4, PRA, Protein S100 A6, S100A6 |
Calculated Molecular Weight | 10 kDa |
Observed Molecular Weight | 10 kDa |
GenBank Accession Number | BC001431 |
Gene Symbol | S100A6 |
Gene ID (NCBI) | 6277 |
RRID | AB_2183801 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P06703 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for S100A6 antibody 10245-1-AP | Download protocol |
IHC protocol for S100A6 antibody 10245-1-AP | Download protocol |
IF protocol for S100A6 antibody 10245-1-AP | Download protocol |
IP protocol for S100A6 antibody 10245-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. | ||
Sci Transl Med Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis. | ||
Cancer Commun (Lond) Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line. | ||
Mol Carcinog S100A6 promotes cell proliferation in human nasopharyngeal carcinoma via the p38/MAPK signalling pathway.
| ||
Neurobiol Aging Genetic screening of ANXA11 revealed novel mutations linked to amyotrophic lateral sclerosis. |