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验证数据展示

  • WB analysis of MCF-7 using 83559-5-RR (same clone as 83559-5-PBS)

    WB analysis of MCF-7 using 83559-5-RR (same clone as 83559-5-PBS)

    WB result of S100A14 antibody (83559-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A14 transfected MCF-7 cells. This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • WB analysis using 83559-5-RR (same clone as 83559-5-PBS)

    WB analysis using 83559-5-RR (same clone as 83559-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83559-5-RR (S100A14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

    IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A14 antibody included
  • IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

    IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A14 antibody included
  • FC experiment of HeLa using 83559-5-RR (same clone as 83559-5-PBS)

    FC experiment of HeLa using 83559-5-RR (same clone as 83559-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83559-5-RR

    Affinity and Kinetic Characterization of 83559-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83559-5-RR against Human S100A14 were performed. The affinity constant is 4.90 nM.

  • Featured Product
  • KD/KO验证

S100A14 Recombinant monoclonal antibody, PBS Only

S100A14 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83559-5-PBS

产品说明书

CloneNo. 240528B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

240528B6, Protein S100 A14, Protein S100-A14, S100 calcium-binding protein A14, S100A15

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy S100A14 Ready-To-Use IHC Kit. S100A14 primary antibody included.

产品信息

83559-5-PBS targets S100A14 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0757

Product name: Recombinant human S100A14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-104 aa of BC005019

Sequence: MGQCRSANAEDAQEFSDVERAIETLIKNFHQYSVEGGKETLTPSELRDLVTQQLPHLMPSNCGLEEKIANLGSCNDSKLEFRSFWELIGEAAKSVKLERPVRGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A14
别名 240528B6, Protein S100 A14, Protein S100-A14, S100 calcium-binding protein A14, S100A15
计算分子量 12 kDa
观测分子量12 kDa
GenBank蛋白编号BC005019
基因名称 S100A14
Gene ID (NCBI) 57402
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9HCY8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).7

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