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验证数据展示

  • WB analysis of A431 using 81017-1-RR (same clone as 81017-1-PBS)

    WB analysis of A431 using 81017-1-RR (same clone as 81017-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 81017-1-RR (S100A10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 81017-1-RR (same clone as 81017-1-PBS)

    IHC staining of human colon using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81017-1-RR (S100A10 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human lung cancer using 81017-1-RR (same clone as 81017-1-PBS)

    IHC staining of human lung cancer using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human lung cancer using 81017-1-RR (same clone as 81017-1-PBS)

    IHC staining of human lung cancer using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A10 antibody included
  • Affinity and Kinetic Characterization of 81017-1-RR

    Affinity and Kinetic Characterization of 81017-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81017-1-RR against Human S100A10 were performed. The affinity constant is 24.5 nM.

    {{ptg:ProductFeaturesInfo}}

S100A10 Recombinant monoclonal antibody, PBS Only

S100A10 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81017-1-PBS

产品说明书

CloneNo. 8G23

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG, ANX2L

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy S100A10 Ready-To-Use IHC Kit. S100A10 primary antibody included.

产品信息

81017-1-PBS targets S100A10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1779

Product name: Recombinant human S100A10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-97 aa of BC015973

Sequence: MPSQMEHAMETMMFTFHKFAGDKGYLTKEDLRVLMEKEFPGFLENQKDPLAVDKIMKDLDQCRDGKVGFQSFFSLIAGLTIACNDYFVVHMKQKGKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A10
别名 Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG, ANX2L
计算分子量 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC015973
基因名称 S100A10
Gene ID (NCBI) 6281
RRIDAB_2918925
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP60903
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.