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S100A10 Monoclonal antibody

S100A10 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B3F9

Cat No : 66227-1-Ig

Print datasheet

Synonyms

42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10

验证数据展示

  • WB analysis of human lung using 66227-1-Ig

    WB analysis of human lung using 66227-1-Ig

    human lung tissue were subjected to SDS PAGE followed by western blot with 66227-1-Ig (S100A10 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 66227-1-Ig

    IHC staining of human colon cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human colon cancer using 66227-1-Ig

    IHC staining of human colon cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A10 antibody included
  • IF Staining of A431 using 66227-1-Ig

    IF Staining of A431 using 66227-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of Saos-2 using 66227-1-Ig

    IF Staining of Saos-2 using 66227-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human stomach cancer using 66227-1-Ig

    IHC staining of human stomach cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human liver cancer using 66227-1-Ig

    IHC staining of human liver cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human liver cancer using 66227-1-Ig

    IHC staining of human liver cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human cervical cancer using 66227-1-Ig

    IHC staining of human cervical cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human cervical cancer using 66227-1-Ig

    IHC staining of human cervical cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A10 antibody included

经过测试的应用

Positive WB detected inhuman lung tissue
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human liver cancer tissue, human stomach cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, Saos-2 cells
Planning an IHC experiment? We recommend our IHCeasy S100A10 Ready-To-Use IHC Kit. S100A10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66227-1-Ig targets S100A10 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human
Immunogen S100A10 fusion protein Ag23596 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name S100 calcium binding protein A10
Synonyms 42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC015973
Gene Symbol S100A10
Gene ID (NCBI) 6281
RRIDAB_2881618
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60903
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

实验方案

Product Specific Protocols
WB protocol for S100A10 antibody 66227-1-IgDownload protocol
IHC protocol for S100A10 antibody 66227-1-IgDownload protocol
IF protocol for S100A10 antibody 66227-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols