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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 67028-1-Ig (same clone as 67028-1-PBS)

    WB analysis using 67028-1-Ig (same clone as 67028-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 67028-1-Ig (same clone as 67028-1-PBS)

    IHC staining of human lymphoma using 67028-1-Ig (same clone as 67028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPL4 antibody included
  • IF Staining of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    IF Staining of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (67028-1-Ig, Clone: 3C7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    IF Staining of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (67028-1-Ig, Clone: 3C7A8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    FC experiment of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (67028-1-Ig, Clone:3C7A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

Ribosomal protein L4 Monoclonal antibody, PBS Only

Ribosomal protein L4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67028-1-PBS

产品说明书

CloneNo. 3C7A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

RPL4, 3C7A8, 60S ribosomal protein L1, 60S ribosomal protein L4, RPL 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RPL4 Ready-To-Use IHC Kit. RPL4 primary antibody included.

产品信息

67028-1-PBS targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Ribosomal protein L4 fusion protein Ag28728 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein L4
别名 RPL4, 3C7A8, 60S ribosomal protein L1, 60S ribosomal protein L4, RPL 4
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC009888
基因名称 Ribosomal protein L4
Gene ID (NCBI) 6124
RRIDAB_2882343
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36578
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.