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验证数据展示

  • WB analysis of Jurkat using 10509-1-Ig (same clone as 10509-1-PBS)

    WB analysis of Jurkat using 10509-1-Ig (same clone as 10509-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10509-1-Ig (RhoGDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10509-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 10509-1-Ig (same clone as 10509-1-PBS)

    WB analysis of HEK-293 using 10509-1-Ig (same clone as 10509-1-PBS)

    WB result of RhoGDI antibody (10509-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RhoGDI transfected HEK-293 cells. This data was developed using the same antibody clone with 10509-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10509-1-Ig (same clone as 10509-1-PBS)

    IHC staining of human breast cancer using 10509-1-Ig (same clone as 10509-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10509-1-Ig(RhoGDI antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10509-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARHGDIA antibody included
  • IHC staining of human breast cancer using 10509-1-Ig (same clone as 10509-1-PBS)

    IHC staining of human breast cancer using 10509-1-Ig (same clone as 10509-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10509-1-Ig(RhoGDI antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10509-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARHGDIA antibody included
  • Featured Product
  • KD/KO Validated

RhoGDI Polyclonal antibody, PBS Only

RhoGDI Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10509-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ARHGDIA Ready-To-Use IHC Kit. ARHGDIA primary antibody included.

产品信息

10509-1-PBS targets RhoGDI in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0791

Product name: Recombinant human ARHGDIA,aGDI protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-204 aa of BC005851

Sequence: MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVVVTGLTLVCSSAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPVEEAPKGMLARGSYSIKSRFTDDDKTDHLSWEWNLTIKKDWKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho GDP dissociation inhibitor (GDI) alpha
别名
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC005851
基因名称 RhoGDI
Gene ID (NCBI) 396
RRIDAB_2059582
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP52565
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.

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