验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, U2OS cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27497-1-AP targets Reticulocalbin 3 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag26569 Product name: Recombinant human RCN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 71-161 aa of BC013436 Sequence: LTPEESQARLGRIVDRMDRAGDGDGWVSLAELRAWIAHTQQRHIRDSVSAAWDTYDTDRDGRVGWEELRNATYGHYAPGEEFHDVEDAETY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | reticulocalbin 3, EF-hand calcium binding domain |
| 别名 | RCN3, Reticulocalbin 3, RLP49 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC013436 |
| 基因名称 | Reticulocalbin 3 |
| Gene ID (NCBI) | 57333 |
| RRID | AB_2880890 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96D15 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Reticulocalbin 3 antibody 27497-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
