验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, A431 cells, HeLa cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25968-1-AP targets Repetin in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Repetin fusion protein Ag23257 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | repetin |
| 别名 | RPTN |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC112216 |
| 基因名称 | RPTN |
| Gene ID (NCBI) | 126638 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6XPR3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Repetin (also known as RPTN), is a member of the "fused" gene family and localized in the epidermal differentiation complex on chromosome 1q21. Repetin contains 2 N-terminal EF hand motifs, a central domain containing 28 repeats of the consensus sequence QxDRQGQSSHYG, and a glutamine- and arginine-rich C-terminal domain(PMID: 15854042). Repetin is an extracellular epidermal matrix protein involved in forming keratinocyte envelope. Repetin is expressed in the epidermis and at high levels in eccrine sweat glands, the inner sheaths of hair roots, and the filiform papilli of the tongue(PMID: 20448178).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Repetin antibody 25968-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |