验证数据展示
经过测试的应用
| Positive WB detected in | Transfected HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28063-1-AP targets Red fluorescent protein (RFP611) in WB, ELISA applications and shows reactivity with parasicyonis actinostoloides, recombinant protein samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | parasicyonis actinostoloides, recombinant protein |
| 免疫原 |
CatNo: Ag27893 Product name: Recombinant entacmaea quadricolor Red fluorescent protein protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-231 aa of Sequence: MNSLIKENMRMMVVMEGSVNGYQFKCTGEGDGNPYMGTQTMRIKVVEGGPLPFAFDILATSFMYGSKTFIKHTKGIPDFFKQSFPEGFTWERVTRYEDGGVFTVMQDTSLEDGCLVYHAKVTGVNFPSNGAVMQKKTKGWEPNTEMLYPADGGLRGYSQMALNVDGGGYLSCSFETTYRSKKTVENFKMPGFHFVDHRLERLEESDKEMFVVQHEHAVAKFCDLPSKLGRL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Red fluorescent protein (RFP611) |
| 别名 | Red fluorescent protein, RFP, Red fluorescent protein eqFP611, GFP-like chromoprotein, eqFP611 |
| 计算分子量 | 26 kDa |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2918144 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8ISF8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is a rabbit polyclonal antibody raised against RFP from Entacmaea quadricolor (eqFP611).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Red fluorescent protein (RFP611) antibody 28063-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |