Rat gp130/IL6ST ELISA Kit
Sensitivity
21.8 pg/mL
Range
46.88-3000 pg/mL
Reactivity
Rat
Cat no : KE20035
Synonyms
CD130, Glycoprotein 130, gp130, IL-6 receptor subunit beta, IL-6R subunit beta
Validation Data Gallery
Product Information
KE20035 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat gp130/IL6ST ELISA kit is to be used to detect and quantify protein levels of endogenous rat gp130/IL6ST. The assay recognizes rat gp130/IL6ST. An antibody specific for rat gp130/IL6ST has been pre-coated onto the microwells. The rat gp130/IL6ST protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for rat gp130/IL6ST is added to detect the captured rat gp130/IL6ST protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat gp130/IL6ST ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 21.8 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 25205 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 90% | 80%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,458.8 | 49.2 | 3.4 |
| 2 | 8 | 427.1 | 12.9 | 3.0 |
| 3 | 8 | 147.7 | 4.5 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,417.2 | 69.2 | 4.9 |
| 2 | 16 | 428.2 | 12.5 | 2.9 |
| 3 | 16 | 149.0 | 4.9 | 3.3 |
Background Information
The interleukin 6 signal transducer (IL6ST, also known as gp130) is a ubiquitously expressed intermediate of the interleukin-6 signaling pathway, which is responsible for signal transduction and stabilization of the α-chain ligand complex. It is a highly pleiotropic shared cytokine signaling receptor that forms oligomeric signaling complexes with ligand binding cytokine receptors. Changes in gp130 activation directly can affect heart functions, such as the hypertrophic response to pressure overload and survival of myocytes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD130, Glycoprotein 130, gp130, IL-6 receptor subunit beta, IL-6R subunit beta |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
