Rat KIM-1/HAVCR1 ELISA Kit

Sensitivity

0.4 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Rat

Cat No : KE20012

说明书下载

Synonyms

CD365, HAVCR1, Kidney injury molecule 1, KIM 1, KIM1, KIM-1, TIM 1, TIM1, TIM-1



Product Information

KE20012 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The KIM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous KIM-1. The assay recognizes rat KIM-1. An antibody specific for KIM-1 has been pre-coated onto the microwells. The KIM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat KIM-1 is added to detect the captured rat KIM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Rat KIM-1/HAVCR1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine
Assay type Sandwich
Sensitivity 0.4 pg/mL
Range 15.6-1000 pg/mL
Reactivity Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 286934

Recovery

Sample TypeAverageRange
Rat Serum116%105%-126%
cell culture supernatants95%82%-105%
rat urine100%84%-116%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120497.29.01.8
220116.31.91.6
32028.50.82.9

InterAssay

Samplenmean ( pg/mL)SDCV%
124439.329.56.7
224107.99.68.9
32426.42.69.8

Background Information

Kidney injury molecule 1 (KIM-1), also known as Hepatitis A virus cellular receptor 1 (HAVCR1) or T-cell immunoglobulin and mucin domain 1 (TIM-1), is a class I integral membrane glycoprotein, with an ectodomain containing Ig-like domain and a mucin domain. KIM-1 acts as a membrane receptor for hepatitis A virus (HAV). KIM-1 provides a costimulatory signal for T cell activation and inhibits the development of peripheral tolerance. KIM-1 may be involved in the regulation of asthma and allergic diseases. It has been reported that KIM-1 is shed into urine after acute kidney damage and is a marker of renal tubular injury.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CD365, HAVCR1, Kidney injury molecule 1, KIM 1, KIM1, KIM-1, TIM 1, TIM1, TIM-1

Publications

SpeciesSample TypeTitle
ratserum

J Ethnopharmacol

Xanthoceras sorbifolium leaves alleviate hyperuricemic nephropathy by inhibiting the PI3K/AKT signaling pathway to regulate uric acid transport

Authors - Yuchao Liu