Rat RAGE ELISA Kit
Validation Data Gallery
Product Information
KE20030 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat RAGE ELISA kit is to be used to detect and quantify protein levels of endogenous rat RAGE. The assay recognizes rat RAGE. An antibody specific for rat RAGE has been pre-coated onto the microwells. The rat RAGE protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for rat RAGE is added to detect the captured rat RAGE protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat RAGE ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 4.7 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 81722 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 104% | 89%-121% |
| Rat urine | 106% | 103%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 540.7 | 20.9 | 3.9 |
| 2 | 20 | 126.1 | 4.7 | 3.8 |
| 3 | 20 | 31.7 | 1.5 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 486.9 | 32.6 | 6.7 |
| 2 | 24 | 120.2 | 6.6 | 5.5 |
| 3 | 24 | 31.0 | 1.3 | 4.3 |
Background Information
RAGE is a multiligand receptor belonging to the immunoglobulin superfamily of cell surface receptors. It is highly expressed in inflammation-related pathological states and is associated with severe chronic pathologies, including diabetic complications, chronic inflammation, and cancer. Soluble forms of RAGE (sRAGE) are produced by proteolytic cleavage of full-length RAGE and alternative mRNA splicing. sRAGE can antagonize full-length RAGE and other receptors by binding DAMPs and other ligands, inhibiting leukocyte recruitment in various acute and chronic inflammatory conditions. sRAGE also has been identified as possessing therapeutic potential, as it can alleviate myocardial fibrosis and suppress autophagy and ischemia/reperfusion injury in rodent studies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AGER,RAGE,Advanced glycosylation end product-speci |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Hum Exp Toxicol Omarigliptin/shikonin combination alleviates cyclosporine-induced nephrotoxicity: The role of sirtuin 1, glucagon-like peptide-1, HMGB1/RAGE/TLR4 signaling, and p38/ERK/JNK MAPKs. | ||
Animal Model Exp Med Metabolic and biochemical profiling reveals phenotypic heterogeneity in Zucker diabetic fatty rats. |
