Rat Lipocalin-2/NGAL ELISA Kit
Sensitivity
1.8 pg/mL
Range
7.8-500 pg/mL
Reactivity
Rat
Validation Data Gallery
Product Information
KE20034 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat Lipocalin-2/NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous rat Lipocalin-2/NGAL. The assay recognizes rat Lipocalin-2/NGAL. An antibody specific for rat Lipocalin-2/NGAL has been pre-coated onto the microwells. The rat Lipocalin-2/NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for rat Lipocalin-2/NGAL is added to detect the captured rat Lipocalin-2/NGAL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat Lipocalin-2/NGAL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.8 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 170496 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 103% | 98%-109% |
| Tissue lysate | 105% | 99%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 136.1 | 2.3 | 1.7 |
| 2 | 20 | 48.7 | 0.7 | 1.5 |
| 3 | 20 | 22.0 | 0.6 | 2.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 136.3 | 3.4 | 2.5 |
| 2 | 24 | 49.0 | 0.7 | 1.5 |
| 3 | 24 | 21.7 | 0.6 | 2.7 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL) also known as Lipocalin-2 (LCN2), is a secreted glycoprotein that belongs to the lipocalin protein family. Several roles of NGAL were identified, including iron trafficking, chemotactic, and promoting cell differentiation and proliferation. NGAL was originally found in neutrophils and expressed in tissues containing renal, uterus, liver, spleen, cardiomyocytes, neurons, and immune cells. NGAL is a significant biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation, and metabolic diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Lcn2,Alpha-2-microglobulin-related protein,Alpha-2 |