Rat IL-2 ELISA Kit
Validation Data Gallery
Product Information
KE20002 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL2. The assay recognizes mouse IL2. An antibody specific for IL2 has been pre-coated onto the microwells. The IL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL2 is added to detect the captured IL2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat IL-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 8.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 116562 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 106% | 93%-119% |
| Rat Serum | 97% | 83%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 38.4 | 2.4 | 6.2 |
| 2 | 20 | 219.4 | 9.8 | 4.5 |
| 3 | 20 | 1,013.1 | 62.1 | 6.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 37.0 | 2.3 | 6.2 |
| 2 | 24 | 225.5 | 17.2 | 7.6 |
| 3 | 24 | 996.8 | 84.6 | 8.5 |
Background Information
Interleukin-2 (IL2) is a protein initially identified as a T cell growth factor. IL2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors. Nevertheless, it has been shown that IL2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (IL2R). IL2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il2, interleukin 2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Evid Based Complement Alternat Med Anti-inflammatory Mechanism of Ruzu Bitters on Diet-Induced Nonalcoholic Fatty Liver Disease in Male Wistar Rats. |
