Speedy™ Rat IL-2 One-Step ELISA Kit
Sensitivity
2.7 pg/mL
Range
7.8-500 pg/mL
Reactivity
Rat
Validation Data Gallery
Product Information
| Product name | Speedy™ Rat IL-2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.7 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 116562 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 111% | 92%-108% |
| Cell culture supernatant | 90% | 74%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 272.5 | 9.0 | 3.3 |
| 2 | 8 | 69.1 | 5.0 | 7.2 |
| 3 | 8 | 34.7 | 1.7 | 4.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 271.8 | 8.1 | 3.0 |
| 2 | 16 | 68.6 | 4.3 | 6.2 |
| 3 | 16 | 34.1 | 2.2 | 6.6 |
Background Information
IL-2 (Interleukin-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It regulates white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. The major sources of IL-2 are activated CD4+ T cells and activated CD8+ T cells. IL-2 mediates its effects by binding to IL-2 receptors which are expressed by lymphocytes. IL-2 signal can be transduced by 3 different signaling pathways: JAK-STAT, PI3K/Akt/mTOR and MAPK/ERK pathway. IL-2 plays a role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. It also promotes proliferation of activated B cells and subsequently immunoglobulin production. IL-2 has been used to treat immune-deficiencies like HIV, and induce immune response against tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il2, interleukin 2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
