Rat IL-1 beta ELISA Kit
Validation Data Gallery
Product Information
KE20021 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat IL-1 beta ELISA kit is to be used to detect and quantify protein levels of endogenous rat IL-1 beta. The assay recognizes rat IL-1 beta. An antibody specific for rat IL-1 beta has been pre-coated onto the microwells. The rat IL-1 beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for rat IL-1 beta is added to detect the captured rat IL-1 beta protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Rat IL-1 beta ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.9 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24494 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat Serum | 114% | 102%-127% |
| Cell culture supernatant | 97% | 70%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 470.7 | 20.4 | 4.3 |
| 2 | 20 | 112.8 | 4.2 | 3.7 |
| 3 | 20 | 28.8 | 1.2 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 493.8 | 38.3 | 7.8 |
| 2 | 24 | 110.0 | 5.4 | 4.9 |
| 3 | 24 | 27.1 | 1.5 | 5.6 |
Background Information
Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il1 beta, Il1b, IL 1 beta, IL-1 beta,IL 1 beta,IL1beta, IL-1 β |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Commun Signal Spinster homolog 2/S1P signaling ameliorates macrophage inflammatory response to bacterial infections by balancing PGE2 production | ||
J Ginseng Res Panax ginseng alleviates diabetic peripheral neuropathy by suppressing mitochondrial dysfunction and oxidative stress via modulation of the RAGE, NF-κB, and Nrf2 pathways. | ||
Int Immunopharmacol Bardoxolone methyl breaks the vicious cycle between M1 macrophages and senescent nucleus pulposus cells through the Nrf2/STING/NF-κB pathway | ||
Biochim Biophys Acta Mol Basis Dis Hyperglycemia and circadian disruption lead to retinal dysfunction in a stabilized colony of the fat sand rat Psammomys obesus | ||
Aging (Albany NY) Medium-chain chlorinated paraffins (MCCPs) induce renal cell aging and ferroptosis | ||
J Ethnopharmacol Omics-based pharmacological evaluation reveals Yuanhu Zhitong oral liquid ameliorates arthritis by regulating PKC/ERK/NF-κB signaling pathway |
