Rat ICAM-1/CD54 ELISA Kit
Sensitivity
3.1 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Rat
Validation Data Gallery
Product Information
KE20013 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ICAM-1/CD54 ELISA kit is to be used to detect and quantify protein levels of endogenous ICAM-1/CD54 . The assay recognizes rat ICAM-1/CD54 . An antibody specific for ICAM-1/CD54 has been pre-coated onto the microwells. The ICAM-1/CD54 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for ICAM-1/CD54 is added to detect the captured ICAM-1/CD54 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat ICAM-1/CD54 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 25464 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 85% | 79%-93% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 547.4 | 13.9 | 2.5 |
| 2 | 20 | 124.0 | 3.3 | 2.6 |
| 3 | 20 | 29.0 | 0.9 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 546.0 | 12.5 | 2.3 |
| 2 | 24 | 124.4 | 2.7 | 2.2 |
| 3 | 24 | 27.6 | 0.7 | 2.4 |
Background Information
Intercellular adhesion molecule 1 (ICAM-1, also known as CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin. It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum. ICAM-1 can exist as membrane-bound form (mICAM-1) and soluble form (sICAM-1). The sICAM-1 arises from alternative splicing and proteolysis of mICAM-1 and appears to be associated with leukocyte activation to produce LFA-1.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD54, ICAM, Icam1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
