Rat CD4 ELISA Kit
Sensitivity
10.4 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Rat
Validation Data Gallery
Product Information
KE20041 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat CD4 ELISA kit is to be used to detect and quantify protein levels of endogenous rat CD4. The assay recognizes rat CD4. An antibody specific for rat CD4 has been pre-coated onto the microwells. The rat CD4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for rat CD4 is added to detect the captured rat CD4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat CD4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 10.4 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24932 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 95% | 85%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,884.6 | 116.7 | 6.2 |
| 2 | 8 | 510.4 | 20.1 | 3.9 |
| 3 | 8 | 155.1 | 6.5 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,975.1 | 162.2 | 8.2 |
| 2 | 16 | 530.8 | 29.4 | 5.5 |
| 3 | 16 | 162.8 | 11.1 | 6.8 |
Background Information
CD4 is a transmembrane glycoprotein of the immunoglobulin superfamily (IgSF), containing four extracellular Ig-like domains, a hydrophobic transmembrane region, and a 40-residue cytoplasmic tail. CD4 is expressed in T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It is involved in T cell differentiation, development, and activation by interacting with antigen-presenting cells (APCs). The cytoplasmic tail of CD4 is associated with the Lck kinase, which in turn activates the signaling components of the T cell receptor (TCR)-CD3 complexes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD4 molecule, p55 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
