Speedy™ Rat AGER/RAGE One-Step ELISA Kit
Sensitivity
3.6 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Rat
Validation Data Gallery
Product Information
| Product name | Speedy™ Rat AGER/RAGE One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 3.6 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 81722 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 91% | 81%-97% |
| Urine | 90% | 86%-93% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,087.7 | 26.9 | 2.5 |
| 2 | 8 | 258.6 | 3.4 | 1.3 |
| 3 | 8 | 146.2 | 2.2 | 1.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,134.4 | 56.6 | 5.0 |
| 2 | 16 | 261.1 | 4.9 | 1.9 |
| 3 | 16 | 149.4 | 4.2 | 2.8 |
Background Information
RAGE is a multiligand receptor belonging to the immunoglobulin superfamily of cell surface receptors. It is highly expressed in inflammation-related pathological states and is associated with severe chronic pathologies, including diabetic complications, chronic inflammation, and cancer. Soluble forms of RAGE (sRAGE) are produced by proteolytic cleavage of full-length RAGE and alternative mRNA splicing. sRAGE can antagonize full-length RAGE and other receptors by binding DAMPs and other ligands, inhibiting leukocyte recruitment in various acute and chronic inflammatory conditions. sRAGE also has been identified as possessing therapeutic potential, as it can alleviate myocardial fibrosis and suppress autophagy and ischemia/reperfusion injury in rodent studies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AGER, RAGE, Advanced glycosylation end product-specific receptor |
FAQ
1、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
