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RanGAP1 Polyclonal antibody

RanGAP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 27405-1-AP

Print datasheet

Synonyms

Fug1, KIAA1835, RANGAP1, SD

验证数据展示

  • WB analysis of SKOV-3 using 27405-1-AP

    WB analysis of SKOV-3 using 27405-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 27405-1-AP

    WB analysis using 27405-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27405-1-AP (RanGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSKOV-3 cells, MCF-7 cells, HEK-293 cells, NIH/3T3 cells, Neuro-2a cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27405-1-AP targets RanGAP1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RanGAP1 fusion protein Ag26128 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ran GTPase activating protein 1
Synonyms Fug1, KIAA1835, RANGAP1, SD
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 64 kDa, 82 kDa
GenBank Accession NumberBC014044
Gene Symbol RANGAP1
Gene ID (NCBI) 5905
RRIDAB_2880863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46060
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.

实验方案

Product Specific Protocols
WB protocol for RanGAP1 antibody 27405-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

Authors - James W Clancy
View Article