验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, Neuro-2a cells, SKOV-3 cells, NIH/3T3 cells | 
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 | 
推荐稀释比
| 应用 | 推荐稀释比 | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 | 
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below | 
| IF | See 1 publications below | 
产品信息
27405-1-AP targets RanGAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description | 
| 文献引用应用 | WB, IF | 
| 经测试反应性 | human, mouse | 
| 文献引用反应性 | human, mouse | 
| 免疫原 | CatNo: Ag26128 Product name: Recombinant human RANGAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-220 aa of BC014044 Sequence: ASEDIAKLAETLAKTQVAGGQLSFKGKSLKLNTAEDAKDVIKEIEDFDSLEALRLEGNTVGVEAARVIAKALEKKSELKRCHWSDMFTGRLRTEIPPALISLGEGLITAGAQLVELDLSDNAFGPDGVQGFEALLKSSACFTLQELKLNNCGMGIGGGKILAAALTECHRKSSAQGKPLALKVFVAGRNRLENDGATALAEAFRVIGTLEEVHMPQNGI种属同源性预测 | 
| 宿主/亚型 | Rabbit / IgG | 
| 抗体类别 | Polyclonal | 
| 产品类型 | Antibody | 
| 全称 | Ran GTPase activating protein 1 | 
| 别名 | SD, Ran GTPase-activating protein 1, KIAA1835 | 
| 计算分子量 | 64 kDa | 
| 观测分子量 | 64 kDa, 82 kDa | 
| GenBank蛋白编号 | BC014044 | 
| 基因名称 | RANGAP1 | 
| Gene ID (NCBI) | 5905 | 
| RRID | AB_2880863 | 
| 偶联类型 | Unconjugated | 
| 形式 | Liquid | 
| 纯化方式 | Antigen affinity purification | 
| UNIPROT ID | P46060 | 
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. | 
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
背景介绍
RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RanGAP1 antibody 27405-1-AP | Download protocol | 
| WB protocol for RanGAP1 antibody 27405-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
发表文章
| Species | Application | Title | 
|---|---|---|
| iScience Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain | 






