验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Ramos cells, Y79 cells |
| Positive IHC detected in | rat liver tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
27606-1-AP targets RanBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag26370 Product name: Recombinant human RANBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2801-2900 aa of NM_006267 Sequence: MKSISSPSVSSETMDKPVDLSTRKEIDTDSTSQGESKIVSFGFGSSTGLSFADLASSNSGDFAFGSKDKNFQWANTGAAVFGTQSVGTQSAGKVGEDEDGS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAN binding protein 2 |
| 别名 | 358 kDa nucleoporin, ANE1, E3 SUMO protein ligase RanBP2, Nucleoporin Nup358, NUP358 |
| 计算分子量 | 358 kDa |
| 观测分子量 | 358 kDa |
| GenBank蛋白编号 | NM_006267 |
| 基因名称 | RANBP2 |
| Gene ID (NCBI) | 5903 |
| RRID | AB_2880923 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49792 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RanBP2 (Ran-binding protein 2) is also named as p270 and NUP358, and belongs to the RanBP2 E3 ligase family. RanBP2 is E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I (PMID:11792325, PMID:22194619). RanBP2 involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates (PMID:7775481). It is component of the nuclear export pathway (PMID:10078529). RanBP2 recruits BICD2 to the nuclear envelope and cytoplasmic stacks of nuclear pore complex known as annulate lamellae during G2 phase of cell cycle (PMID:20386726).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RanBP2 antibody 27606-1-AP | Download protocol |
| WB protocol for RanBP2 antibody 27606-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol YTHDF1 Aggravates the Progression of Cervical Cancer Through m6A-Mediated Up-Regulation of RANBP2.
| ||
Nat Phys Structural anisotropy results in mechano-directional transport of proteins across nuclear pores
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