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验证数据展示

  • WB analysis using 27804-1-AP (same clone as 27804-1-PBS)

    WB analysis using 27804-1-AP (same clone as 27804-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

  • WB analysis using 27804-1-AP (same clone as 27804-1-PBS)

    WB analysis using 27804-1-AP (same clone as 27804-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 27804-1-AP (same clone as 27804-1-PBS)

    IHC staining of mouse testis using 27804-1-AP (same clone as 27804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27804-1-AP (RanBP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RANBP1 antibody included
  • IF Staining of HeLa using 27804-1-AP (same clone as 27804-1-PBS)

    IF Staining of HeLa using 27804-1-AP (same clone as 27804-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 27804-1-AP (same clone as 27804-1-PBS)

    IF Staining of NIH/3T3 using 27804-1-AP (same clone as 27804-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

RanBP1 Polyclonal antibody, PBS Only

RanBP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27804-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RANBP1 Ready-To-Use IHC Kit. RANBP1 primary antibody included.

产品信息

27804-1-PBS targets RanBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25714

Product name: Recombinant human RANBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-60 aa of

Sequence: MLNAENAQKFKTKFEECRKEIEEREKKAGSGKNDHAEKVAEKLEALSVKEETKEDAEEKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAN binding protein 1
别名
观测分子量 28 kDa
基因名称 RANBP1
Gene ID (NCBI) 5902
RRIDAB_2880977
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43487
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RanBP1 (Ran-binding protein 1) belongs to the RANBP1 family. RanBP1 plays a role in RAN-dependent nucleocytoplasmic transport. It alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus. And RanBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PMID:20485264). RanBP1 promotes dissociation of RAN from a complex with KPNA2 and CSE1L. It required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PMID:17671426). Also, RanBP1 inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PMID:7882974, PMID:7616957).

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