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验证数据展示

  • WB analysis using 11126-1-AP

    WB analysis using 11126-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11126-1-AP (Rad51D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11126-1-AP

    IHC staining of human lymphoma using 11126-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11126-1-AP (Rad51D antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of mouse testis using 11126-1-AP

    IHC staining of mouse testis using 11126-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11126-1-AP (Rad51D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Rad51D Polyclonal antibody

Rad51D Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11126-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

HsTRAD, R51H3, RAD51 homolog D, RAD51 like 3 (S. cerevisiae), RAD51 like protein 3, Rad51D, RAD51L3, Trad

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, A2780 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11126-1-AP targets Rad51D in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1595

Product name: Recombinant human Rad51D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-253 aa of BC014422

Sequence: MGVLRVGLCPGLTEEMIQLLRSHRIKTVVDLVSADLEEVAQKCGLSYKALVALRRVLLAQFSAFPVNGADLYEELKTSTAILSTGIGSLDKLLDAGLYTGEVTEIVGGPGSGKTQVCLCMAANVAHGLQQNVLYVDSNGGLTASRLLQLLQAKTQDEEEQAEALRRIQVVHAFDIFQMLDVLQELRGTVAQQVTGSSGTVKVVVVDSVTAVVSPLLGGQQREGLALMMQLARELKTLARDLGMAVVVTNHITR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAD51-like 3 (S. cerevisiae)
别名 HsTRAD, R51H3, RAD51 homolog D, RAD51 like 3 (S. cerevisiae), RAD51 like protein 3, Rad51D, RAD51L3, Trad
计算分子量 35 kDa
观测分子量 30-40 kDa
GenBank蛋白编号BC014422
基因名称 Rad51D
Gene ID (NCBI) 5892
RRIDAB_10644480
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75771
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD51L3, also named RAD51D, R51H3 and TRAD, belongs to the recA family and RAD51 subfamily. It is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA, and specifically to nicks in duplex DNA. The Heterodimer of RAD51L3 is about 70 kDa.

实验方案

Product Specific Protocols
IHC protocol for Rad51D antibody 11126-1-APDownload protocol
WB protocol for Rad51D antibody 11126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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