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RXRB Polyclonal antibody

RXRB Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14684-1-AP

Print datasheet

Synonyms

DAUDI6, H 2RIIBP, NR2B2, RCoR 1, Retinoid X receptor beta, retinoid X receptor, beta, RXRB

验证数据展示

  • IHC staining of human colon cancer using 14684-1-AP

    IHC staining of human colon cancer using 14684-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14684-1-AP (RXRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RXRB antibody included
  • WB analysis of NIH/3T3 using 14684-1-AP

    WB analysis of NIH/3T3 using 14684-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14684-1-AP (RXRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14684-1-AP

    IHC staining of human colon cancer using 14684-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14684-1-AP (RXRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RXRB antibody included
  • IP experiment of MCF-7 using 14684-1-AP

    IP experiment of MCF-7 using 14684-1-AP

    IP result of anti-RXRB (IP:14684-1-AP, 4ug; Detection:14684-1-AP 1:500) with MCF-7 cells lysate 1200ug.

经过测试的应用

Positive WB detected inNIH/3T3 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RXRB Ready-To-Use IHC Kit. RXRB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

14684-1-AP targets RXRB in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen RXRB fusion protein Ag0263 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinoid X receptor, beta
Synonyms DAUDI6, H 2RIIBP, NR2B2, RCoR 1, Retinoid X receptor beta, retinoid X receptor, beta, RXRB
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa, 70 kDa
GenBank Accession NumberBC001167
Gene Symbol RXRB
Gene ID (NCBI) 6257
RRIDAB_10858228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Retinoid X Receptor beta (RXRB) is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid. RXRB heterodimerizes with other nuclear hormone receptors, such as RAR, the thyroid hormone receptor and the vitamin D3 receptor, to enhance binding to their cognate response elements. (PMID: 8257090)

实验方案

Product Specific Protocols
WB protocol for RXRB antibody 14684-1-APDownload protocol
IHC protocol for RXRB antibody 14684-1-APDownload protocol
IP protocol for RXRB antibody 14684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Am J Physiol Lung Cell Mol Physiol

Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia.

Authors - Craig A Gelfand
View Article