验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RXRB Ready-To-Use IHC Kit. RXRB primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
14684-1-AP targets RXRB in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 | RXRB fusion protein Ag0263 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinoid X receptor, beta |
| 别名 | DAUDI6, H 2RIIBP, NR2B2, RCoR 1, Retinoid X receptor beta, retinoid X receptor, beta, RXRB |
| 计算分子量 | 57 kDa |
| 观测分子量 | 57 kDa, 70 kDa |
| GenBank蛋白编号 | BC001167 |
| 基因名称 | RXRB |
| Gene ID (NCBI) | 6257 |
| RRID | AB_10858228 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28702 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Retinoid X Receptor beta (RXRB) is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid. RXRB heterodimerizes with other nuclear hormone receptors, such as RAR, the thyroid hormone receptor and the vitamin D3 receptor, to enhance binding to their cognate response elements. (PMID: 8257090)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RXRB antibody 14684-1-AP | Download protocol |
| IHC protocol for RXRB antibody 14684-1-AP | Download protocol |
| IP protocol for RXRB antibody 14684-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Physiol Lung Cell Mol Physiol Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia. | ||
Exp Mol Med LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver |
