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验证数据展示

  • WB analysis using 21218-1-AP (same clone as 21218-1-PBS)

    WB analysis using 21218-1-AP (same clone as 21218-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 21218-1-AP (same clone as 21218-1-PBS)

    IHC staining of human kidney using 21218-1-AP (same clone as 21218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RXRA antibody included
  • IF Staining of HeLa using 21218-1-AP (same clone as 21218-1-PBS)

    IF Staining of HeLa using 21218-1-AP (same clone as 21218-1-PBS)

    Immunofluorescent analysis of Hela cells, using RXRA antibody 21218-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

  • IP experiment of HCT 116 using 21218-1-AP (same clone as 21218-1-PBS)

    IP experiment of HCT 116 using 21218-1-AP (same clone as 21218-1-PBS)

    HCT 116 lysates prepared and IP of RXRA performed using 1.0 μg of 21218-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 21218-1-AP (same clone as 21218-1-PBS)

    IP experiment of MCF-7 using 21218-1-AP (same clone as 21218-1-PBS)

    IP result of anti-RXRA (IP:21218-1-AP, 4ug; Detection:21218-1-AP 1:600) with MCF-7 cells lysate 800ug. This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 21218-1-AP (same clone as 21218-1-PBS)

    IHC staining of mouse heart using 21218-1-AP (same clone as 21218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RXRA antibody included
  • Featured Product
  • KD/KO Validated

RXRA Polyclonal antibody, PBS Only

RXRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21218-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RXRA Ready-To-Use IHC Kit. RXRA primary antibody included.

产品信息

21218-1-PBS targets RXRA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15651

Product name: Recombinant human RXRA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 113-462 aa of BC110998

Sequence: LNGVLKVPAHPSGNMASFTKHICAICGDRSSGKHYGVYSCEGCKGFFKRTVRKDLTYTCRDNKDCLIDKRQRNRCQYCRYQKCLAMGMKREAVQEERQRGKDRNENEVESTSSANEDMPVERILEAELAVEPKTETYVEANMGLNPSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSIAVKDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIVLFNPDSKGLSNPAEVEALREKVYASLEAYCKHKYPEQPGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLEAPHQMT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinoid X receptor, alpha
别名
计算分子量 462 aa, 51 kDa
观测分子量 50-54 kDa
GenBank蛋白编号BC110998
基因名称 RXRA
Gene ID (NCBI) 6256
RRIDAB_10693633
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19793
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver and also expressed in the lungs, kidneys, and heart. It can recognize the mature 54 kDa RXRA and the truncated 44 kDa RXRA (PMID: 20541701).

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