验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse thymus tissue, HeLa cells, Jurkat cells, rat thymus tissue |
| Positive IP detected in | mouse thymus tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
17036-1-AP targets RWDD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | RWDD1 fusion protein Ag10525 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RWD domain containing 1 |
| 别名 | CGI 24, DFRP2, PTD013, RWD domain containing 1, RWDD1 |
| 计算分子量 | 243 aa, 28 kDa |
| 观测分子量 | 28-36 kDa |
| GenBank蛋白编号 | BC015802 |
| 基因名称 | RWDD1 |
| Gene ID (NCBI) | 51389 |
| RRID | AB_2184704 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H446 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RWDD1(RWD domain-containing protein 1) is also named as DFRP2(DRG family-regulatory protein 2) and belongs to the RWDD1/GIR2 family. RWDD1 is a thymus aging-related protein which contains an RWD domain at its N-terminus and it is expressed in both thymocytes and thymic epithelial cells and located primarily in the cytoplasm. This protein expression in thymus is decreased in aged and oxidatively stressed mice (PMID:18954556). RWDD1 has a calculated molecular mass of 28 kDa, and apparent molecular mass of 32-36 kDa due to its high acidic amino acid composition that results in the slowed migration behavior in electrophoresis (PMID: 30278355, 18954556).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RWDD1 antibody 17036-1-AP | Download protocol |
| IHC protocol for RWDD1 antibody 17036-1-AP | Download protocol |
| IP protocol for RWDD1 antibody 17036-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
