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RUNX1T1 Monoclonal antibody

RUNX1T1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G2F7

Cat No : 67086-1-Ig

Print datasheet

Synonyms

AML1T1, CBFA2T1, CDR, Cyclin D related protein, Eight twenty one protein, ETO, MTG8, MTG8b, Protein CBFA2T1, Protein ETO, Protein MTG8, RUNX1T1, ZMYND2

验证数据展示

  • WB analysis of HEK-293 using 67086-1-Ig

    WB analysis of HEK-293 using 67086-1-Ig

    WB result of RUNX1T1 antibody (67086-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RUNX1T1 transfected HEK-293 cells.

  • WB analysis of Jurkat using 67086-1-Ig

    WB analysis of Jurkat using 67086-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, pig brain tissue, K-562 cells, rat brain tissue, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy RUNX1T1 Ready-To-Use IHC Kit. RUNX1T1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67086-1-Ig targets RUNX1T1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse, rat, pig
Immunogen RUNX1T1 fusion protein Ag7893 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
Synonyms AML1T1, CBFA2T1, CDR, Cyclin D related protein, Eight twenty one protein, ETO, MTG8, MTG8b, Protein CBFA2T1, Protein ETO, Protein MTG8, RUNX1T1, ZMYND2
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC005850
Gene Symbol RUNX1T1
Gene ID (NCBI) 862
RRIDAB_2882394
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ06455
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. Various transcript of the fusion gene has been reported. RUNX1T1 exists some isoforms with MV 68, 67,64, 48 and 44 kDa. The calcualted molecular weight of RUNX1T1 is 67 kDa, but modified RUNX1T1is about 70-75 kDa.

实验方案

Product Specific Protocols
WB protocol for RUNX1T1 antibody 67086-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols