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RUNDC3B Polyclonal antibody

RUNDC3B Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17113-1-AP

Print datasheet

Synonyms

Rap2 binding protein 9, Rap2 interacting protein 9, RPIB9, RPIP 9, RPIP9, RUN domain containing 3B, RUNDC3B

验证数据展示

  • WB analysis of MCF-7 using 17113-1-AP

    WB analysis of MCF-7 using 17113-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17113-1-AP (RUNDC3B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 17113-1-AP

    IF Staining of MCF-7 using 17113-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17113-1-AP (RUNDC3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF7 cells, mouse brain tissue
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17113-1-AP is RUNDC3B Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen RUNDC3B fusion protein Ag10797 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RUN domain containing 3B
Synonyms Rap2 binding protein 9, Rap2 interacting protein 9, RPIB9, RPIP 9, RPIP9, RUN domain containing 3B, RUNDC3B
Calculated Molecular Weight 387 aa, 43 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC022520
Gene Symbol RUNDC3B
Gene ID (NCBI) 154661
RRIDAB_10699872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NL0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RUNDC3B antibody 17113-1-APDownload protocol
IF protocol for RUNDC3B antibody 17113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols