验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31232-1-AP targets RTTN in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34960 Product name: Recombinant human RTTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1417-1530 aa of NM_173630 Sequence: TVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | rotatin |
| 别名 | Rotatin, MSSP |
| 计算分子量 | 249 kDa |
| 观测分子量 | 246 kDa |
| GenBank蛋白编号 | NM_173630 |
| 基因名称 | RTTN |
| Gene ID (NCBI) | 25914 |
| RRID | AB_3742412 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q86VV8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RTTN, also known as Rotatin, is an evolutionarily conserved protein that is associated with the centrosome and plays a crucial role in centriole biogenesis. It is found in many organisms and was initially identified in a mutant mouse that exhibited defects in axial rotation and left-right specification. RTTN is required for proper mitotic progression, the maintenance of spindle pole integrity, and correct spindle positioning. Loss of RTTN can lead to mitotic defects, including G2/M phase arrest and increased aneuploidy cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RTTN antibody 31232-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
