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验证数据展示

  • WB analysis using 15636-1-AP

    WB analysis using 15636-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15636-1-AP (RTN4IP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15636-1-AP

    IF Staining of HepG2 using 15636-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTN4IP1 antibody (15636-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 15636-1-AP

    IF Staining of HepG2 using 15636-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTN4IP1 antibody (15636-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

RTN4IP1 Polyclonal antibody

RTN4IP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15636-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

NIMP, NOGO-interacting mitochondrial protein, Reticulon-4-interacting protein 1, mitochondrial

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15636-1-AP targets RTN4IP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8100

Product name: Recombinant human RTN4IP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-396 aa of BC006399

Sequence: MEFLKTCVLRRNACTAVCFWRSKVVQKPSVRRISTTSPRSTVMPAWVIDKYGKNEVLRFTQNMMMPIIHYPNEVIVKVHAASVNPIDVNMRSGYGATALNMKRDPLHVKIKGEEFPLTLGRDVSGVVMECGLDVKYFKPGDEVWAAVPPWKQGTLSEFVVVSGNEVSHKPKSLTHTQAASLPYVALTAWSAINKVGGLNDKNCTGKRVLILGASGGVGTFAIQVMKAWDAHVTAVCSQDASELVRKLGADDVIDYKSGSVEEQLKSLKPFDFILDNVGGSTETWAPDFLKKWSGATYVTLVTPFLLNMDRLGIADGMLQTGVTVGSKALKHFWKGVHYRWAFFMASGPCLDDIAELVDAGKIRPVIEQTFPFSKVPEAFLKVERGHARGKTVINVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 reticulon 4 interacting protein 1
别名 NIMP, NOGO-interacting mitochondrial protein, Reticulon-4-interacting protein 1, mitochondrial
计算分子量 396 aa, 44 kDa
观测分子量32-44 kDa, 24 kDa
GenBank蛋白编号BC006399
基因名称 RTN4IP1
Gene ID (NCBI) 84816
RRIDAB_3669217
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWV3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RTN4IP1 (Reticulon-4-interacting protein 1, mitochondrial), also known as NIMP. It colocalizes with the endoplasmic reticulum HSPA5 at spots corresponding to contacts with mitochondria (PMID: 26593267), which is widely expressed in mitochondria-enriched tissues. Found in heart, muscle, kidney, liver, brain and placenta (PMID: 12067236). The protein plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. Appears to be a potent inhibitor of regeneration following spinal cord injury. The protein has three isoforms with molecular weights of 43 kDa, 32 kDa and 24 kDa respectively.

实验方案

Product Specific Protocols
IF protocol for RTN4IP1 antibody 15636-1-APDownload protocol
WB protocol for RTN4IP1 antibody 15636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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