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验证数据展示

  • WB analysis using 86162-1-RR

    WB analysis using 86162-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86162-1-RR (RTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 86162-1-RR

    IHC staining of human ovary cancer using 86162-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86162-1-RR (RTN2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 86162-1-RR

    IHC staining of mouse skeletal muscle using 86162-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 86162-1-RR (RTN2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RTN2 Recombinant antibody

RTN2 Recombinant Antibody for WB, IHC, ELISA
Cat No. 86162-1-RR

产品说明书

CloneNo. 250823C1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Neuroendocrine-specific protein-like 1, Neuroendocrine-specific protein-like I, NSPL1, NSPLI, NSP-like protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells, K-562 cells, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle
Positive IHC detected inhuman ovary cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86162-1-RR targets RTN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RTN2 fusion protein Ag1655 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 reticulon 2
别名 Neuroendocrine-specific protein-like 1, Neuroendocrine-specific protein-like I, NSPL1, NSPLI, NSP-like protein 1
计算分子量 59 kDa, 51 kDa, 22 kDa
观测分子量 20-23 kDa
GenBank蛋白编号BC014244
基因名称 RTN2
Gene ID (NCBI) 6253
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75298
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN2 gene produces three isoforms termed RTN2-A, RTN2-B, and RTN2-C. Isoform RTN2-C has a calculated molecular weight of 22 kDa and is highly expressed in skeletal muscle.

实验方案

Product Specific Protocols
WB protocol for RTN2 antibody 86162-1-RRDownload protocol
IHC protocol for RTN2 antibody 86162-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols