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RTN1 (Isoform RTN1-C) Polyclonal antibody

RTN1 (Isoform RTN1-C) Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15048-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

NSP, NSP-C, reticulon 1, RTN1, RTN1 (Isoform RTN1-C), RTN1-C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue, human brain tissue, human placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15048-1-AP targets RTN1 (Isoform RTN1-C) in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 RTN1 (Isoform RTN1-C) fusion protein Ag7031 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 reticulon 1
别名 NSP, NSP-C, reticulon 1, RTN1, RTN1 (Isoform RTN1-C), RTN1-C
计算分子量 84 kDa, 39 kDa, 24 kDa
观测分子量 23 kDa
GenBank蛋白编号BC000314
基因名称 RTN1-C
Gene ID (NCBI) 6252
RRIDAB_2185981
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16799
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN1 (also known as NSP) gene is expressed predominantly in neuroendocrine tissues, and produces three isoforms termed RTN1-A (NSP-A), RTN1-B (NSP-B), and RTN1-C (NSP-C). This antibody is raised against the full-length protein of human RTN1-C.

实验方案

Product Specific Protocols
WB protocol for RTN1 (Isoform RTN1-C) antibody 15048-1-APDownload protocol
IHC protocol for RTN1 (Isoform RTN1-C) antibody 15048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Combinatorial selective ER-phagy remodels the ER during neurogenesis

Authors - Melissa J Hoyer
mouse, ratWB, IF

Cell Death Dis

RTN1-C mediates cerebral ischemia/reperfusion injury via ER stress and mitochondria-associated apoptosis pathways.

Authors - Lingli Gong
  • KD Validated
mouseWB

Hum Mol Genet

Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

Authors - Peng-Peng Zhu
rat,mouseWB

Acta Biochim Biophys Sin (Shanghai)

RTN1-C mediates cerebral ischemia/reperfusion injury via modulating autophagy.

Authors - Jun Ling
  • KD Validated
mouseWB

NPJ Parkinsons Dis

Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities

Authors - Jia Yu
mouseWB

Brain Res Bull

RTN1-C is involved in high glucose-aggravated neuronal cell subjected to oxygen-glucose deprivation and reoxygenation injury via endoplasmic reticulum stress.

Authors - Muya Lin
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