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验证数据展示

  • WB analysis of A431 using 12762-1-AP

    WB analysis of A431 using 12762-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12762-1-AP (RTKN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12762-1-AP

    IHC staining of human prostate cancer using 12762-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12762-1-AP (RTKN antibody) at dilution of 1:100 (under 10x lens).

RTKN Polyclonal antibody

RTKN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12762-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

rhotekin, RTKN, RTKN1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12762-1-AP targets RTKN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RTKN fusion protein Ag3265 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 rhotekin
别名 rhotekin, RTKN, RTKN1
计算分子量 550 aa, 61 kDa
观测分子量 63 kDa
GenBank蛋白编号BC017727
基因名称 RTKN
Gene ID (NCBI) 6242
RRIDAB_10644159
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BST9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RTKN antibody 12762-1-APDownload protocol
IHC protocol for RTKN antibody 12762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols