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RTKN Polyclonal antibody

RTKN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12762-1-AP

Print datasheet

Synonyms

rhotekin, RTKN, RTKN1

验证数据展示

  • WB analysis of A431 using 12762-1-AP

    WB analysis of A431 using 12762-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12762-1-AP (RTKN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12762-1-AP

    IHC staining of human prostate cancer using 12762-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12762-1-AP (RTKN antibody) at dilution of 1:100 (under 10x lens).

经过测试的应用

Positive WB detected inA431 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12762-1-AP targets RTKN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen RTKN fusion protein Ag3265 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name rhotekin
Synonyms rhotekin, RTKN, RTKN1
Calculated Molecular Weight 550 aa, 61 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC017727
Gene Symbol RTKN
Gene ID (NCBI) 6242
RRIDAB_10644159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BST9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RTKN antibody 12762-1-APDownload protocol
IHC protocol for RTKN antibody 12762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols