验证数据展示
经过测试的应用
| Positive WB detected in | BGC-823 cells, C6 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse testis tissue, PC-3 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
产品信息
25337-1-AP targets RTEL1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, zebrafish |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | regulator of telomere elongation helicase 1 |
| 别名 | Novel helicase like, NHL, KIAA1088, EC:3.6.4.12, C20orf41 |
| 计算分子量 | 142 kDa |
| 观测分子量 | 133 kDa |
| GenBank蛋白编号 | NM_016434 |
| 基因名称 | RTEL1 |
| Gene ID (NCBI) | 51750 |
| RRID | AB_2880034 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZ71 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RTEL1 antibody 25337-1-AP | Download protocol |
| IP protocol for RTEL1 antibody 25337-1-AP | Download protocol |
| WB protocol for RTEL1 antibody 25337-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing. | ||
Cell Genom NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors
| ||
J Clin Invest CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes | ||
Cell Rep A multimodal screening platform for endogenous dipeptide repeat proteins in C9orf72 patient iPSC neurons |
