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验证数据展示

  • WB analysis using 15996-1-AP

    WB analysis using 15996-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15996-1-AP (RTCD1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15996-1-AP

    IHC staining of mouse testis using 15996-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15996-1-AP (RTCD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 15996-1-AP

    IHC staining of mouse testis using 15996-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15996-1-AP (RTCD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

RTCD1 Polyclonal antibody

RTCD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15996-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

RNA 3 phosphate cyclase, RNA cyclase, RPC, RPC1, RTC1, RTCD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, Jurkat cells, K-562 cells, THP-1 cells, U-251 cells, U-87 MG cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15996-1-AP targets RTCD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag8904

Product name: Recombinant human RTCD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-379 aa of BC012604

Sequence: MAGPRVEVDGSIMEGGGQILRVSTALSCLLGLPSRVQKIRAGRSTPGLSSGGWKSKIKVLTRPQHLSGLEMIRDLCDGQLEGAEIGSTEITFTPEKIKGGIHTADTKTAGSVCLLMQVSMPCVLFAASPSELHLKGGTNAEMAPQIDYTVMVFKPIVEKFGFIFNCDIKTRGYYPKGGGEVIVRMSPVKQLNPINLTERGCVTKIYGRAFVAGVLPFKVAKDMAAAAVRCIRKEIRDLYVNIQPVQEPKDQAFGNGNGIIIIAETSTGCLFAGSSLGKRGVNADKVGIEAAEMLLANLRHGGTVDEYLQDQLIVFMALANGVSRIKTGPVTLHTQTAIHFAEQIAKAKFIVKKSEDEEDAAKDTYIIECQGIGMTNPNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA terminal phosphate cyclase domain 1
别名 RNA 3 phosphate cyclase, RNA cyclase, RPC, RPC1, RTC1, RTCD1
计算分子量 379 aa, 41 kDa
观测分子量 41-43 kDa
GenBank蛋白编号BC012604
基因名称 RTCD1
Gene ID (NCBI) 8634
RRIDAB_2180923
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00442
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RTCD1 antibody 15996-1-APDownload protocol
IHC protocol for RTCD1 antibody 15996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.

Authors - Irem Unlu
  • KD Validated
View Article