验证数据展示
经过测试的应用
| Positive WB detected in | mouse trachea tissue, rat trachea tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21931-1-AP targets RSPH4A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RSPH4A fusion protein Ag16551 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | radial spoke head 4 homolog A (Chlamydomonas) |
| 别名 | RSHL3, CILD11 |
| 计算分子量 | 716 aa, 81 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC106949 |
| 基因名称 | RSPH4A |
| Gene ID (NCBI) | 345895 |
| RRID | AB_3669381 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5TD94 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RSPH4A, also known as RSHL3, belongs to the radial spoke family. RSPH4A is the component of the axonemal radial spoke head which plays an important role in ciliary motility. Mutations in RSPH4A cause primary ciliary dyskinesia 1, a disease arising from dysmotility of motile cilia and sperm(PMID: 19200523, 23798057).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RSPH4A antibody 21931-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |