验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, NIH/3T3 cells |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17603-1-AP targets RSPH3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, CoIP, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | RSPH3 fusion protein Ag11707 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | radial spoke 3 homolog (Chlamydomonas) |
别名 | RSP3, RSHL2, Radial spoke head-like protein 2, A-kinase anchor protein RSPH3 |
计算分子量 | 560 aa, 64 kDa |
观测分子量 | 70 kDa |
GenBank蛋白编号 | BC050604 |
基因名称 | RSPH3 |
Gene ID (NCBI) | 83861 |
RRID | AB_2181073 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q86UC2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for RSPH3 antibody 17603-1-AP | Download protocol |
IHC protocol for RSPH3 antibody 17603-1-AP | Download protocol |
IF protocol for RSPH3 antibody 17603-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Curr Biol CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement | ||
J Mol Cell Biol Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat. | ||
Andrology Whole-exome sequencing identifies ADGB as a novel causative gene for male infertility in humans: from motility to fertilization | ||
Sci Rep Loss of Rsph9 causes neonatal hydrocephalus with abnormal development of motile cilia in mice. | ||
J Assist Reprod Genet Patients with severe asthenoteratospermia carrying SPAG6 or RSPH3 mutations have a positive pregnancy outcome following intracytoplasmic sperm injection. | ||
Nat Commun Multi-scale structures of the mammalian radial spoke and divergence of axonemal complexes in ependymal cilia |