验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, NIH/3T3 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17603-1-AP targets RSPH3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, CoIP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag11707 Product name: Recombinant human RSPH3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 216-560 aa of BC050604 Sequence: SLELQRQREARKRALARKQAQEQLRPQTPEPVEGRKHVDVQTELYLEEIADRIIEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILEGELFDFDLEVKPVLEVLVGKTIEQSLLEVMEEEELANLRASQREYEELRNSERAEVQRLEEQERRHREEKERRKKQQWEIMHKHNETSQKIAARAFAQRYLADLLPSVFGSLRDSGYFYDPIERDIEIGFLPWLMNEVEKTMEYSMVGRTVLDMLIREVVEKRLCMYEHGEDTHQSPEPEDEPGGPGAMTESLEASEFLEQSMSQTRELLLDGGYLQRTTYDRRSSQERKFMEERELLGQDEETAMRKSLGEEELS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | radial spoke 3 homolog (Chlamydomonas) |
| 别名 | RSP3, RSHL2, Radial spoke head-like protein 2, A-kinase anchor protein RSPH3 |
| 计算分子量 | 560 aa, 64 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC050604 |
| 基因名称 | RSPH3 |
| Gene ID (NCBI) | 83861 |
| RRID | AB_2181073 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86UC2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RSPH3 antibody 17603-1-AP | Download protocol |
| IHC protocol for RSPH3 antibody 17603-1-AP | Download protocol |
| WB protocol for RSPH3 antibody 17603-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement | ||
J Mol Cell Biol Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat. | ||
Andrology Whole-exome sequencing identifies ADGB as a novel causative gene for male infertility in humans: from motility to fertilization | ||
Sci Rep Loss of Rsph9 causes neonatal hydrocephalus with abnormal development of motile cilia in mice. | ||
J Assist Reprod Genet Patients with severe asthenoteratospermia carrying SPAG6 or RSPH3 mutations have a positive pregnancy outcome following intracytoplasmic sperm injection. | ||
Nat Commun Multi-scale structures of the mammalian radial spoke and divergence of axonemal complexes in ependymal cilia |
