验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue |
Planning an IHC experiment? We recommend our IHCeasy RSPH1 Ready-To-Use IHC Kit. RSPH1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32033-1-AP targets RSPH1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | RSPH1 fusion protein Ag36560 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | radial spoke head 1 homolog (Chlamydomonas) |
| 别名 | Radial Spoke Head Component 1, Radial spoke head 1 homolog, Meichroacidin, Male meiotic metaphase chromosome-associated acidic protein, CT79 |
| 计算分子量 | 309 aa, 35 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC101519 |
| 基因名称 | RSPH1 |
| Gene ID (NCBI) | 89765 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8WYR4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RSPH1 (Radial Spoke Head Component 1) encodes a male mid-meiotic chromosome-associated acidic protein involved in the structure and function of cilia and flagella. Pathogenic variants of RSPH1 lead to impaired spermatocyte motility and aberrant flagellar composition, which further contribute to male infertility. In addition to this, mutations in the RSPH1 gene lead to primary ciliary dyskinesia-24 (PCD-24), an autosomal recessive disorder that affects the motor function of cilia and flagella.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RSPH1 antibody 32033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |