验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HL-60 cells, PC-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 3 publications below |
产品信息
25190-1-AP targets RSL24D1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | RSL24D1 fusion protein Ag18195 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal L24 domain containing 1 |
| 别名 | C15orf15, HRP L30 iso, L30, My024, Ribosomal L24 domain-containing protein 1 |
| 计算分子量 | 163 aa, 20 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC008409 |
| 基因名称 | RSL24D1 |
| Gene ID (NCBI) | 51187 |
| RRID | AB_2879950 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHA3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RSL24D1 (Ribosomal L24 Domain Containing 1) is a ribosomal protein that is primarily localized in the nucleolus and is involved in the biogenesis of the 60S ribosomal subunit. In mouse embryonic stem cells (ESCs), the expression level of RSL24D1 is high and significantly decreases during cell differentiation. The role of RSL24D1 in ribosome biogenesis includes facilitating proper protein docking in nucleolar and cytoplasmic precursor particles. After the completion of biogenesis, RSL24D1 dissociates from the particles and is thought to be replaced by ribosomal protein L24.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RSL24D1 antibody 25190-1-AP | Download protocol |
| IF protocol for RSL24D1 antibody 25190-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Labeling of heterochronic ribosomes reveals C1ORF109 and SPATA5 control a late step in human ribosome assembly. | ||
mBio Genome-Scale CRISPR Screening Reveals Host Factors Required for Ribosome Formation and Viral Replication | ||
Cell The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity | ||
Nat Cell Biol A programmed decline in ribosome levels governs human early neurodevelopment |
