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验证数据展示

  • WB analysis using 21877-1-AP

    WB analysis using 21877-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21877-1-AP (RSK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-12 using 21877-1-AP

    IP experiment of PC-12 using 21877-1-AP

    IP result of anti-RSK4 (IP:21877-1-AP, 4ug; Detection:21877-1-AP 1:800) with PC-12 cells lysate 1560 ug.

  • IP experiment of MCF-7 using 21877-1-AP

    IP experiment of MCF-7 using 21877-1-AP

    IP result of anti-RSK4 (IP:21877-1-AP, 4ug; Detection:21877-1-AP 1:800) with MCF-7 cells lysate 1520 ug.

RSK4 Polyclonal antibody

RSK4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 21877-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

RPS6KA6, p90RSK6, pp90RSK4, S6K alpha 6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inMCF-7 cells, PC-12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21877-1-AP targets RSK4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16299

Product name: Recombinant human RPS6KA6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 629-745 aa of BC148802

Sequence: TPEEILLRIGNGKFSLSGGNWDNISDGAKDLLSHMLHMDPHQRYTAEQILKHSWITHRDQLPNDQPKRNDVSHVVKGAMVATYSALTHKTFQPVLEPVAASSLAQRRSMKKRTSTGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S6 kinase, 90kDa, polypeptide 6
别名 RPS6KA6, p90RSK6, pp90RSK4, S6K alpha 6
计算分子量 745 aa, 84 kDa
观测分子量84 kDa
GenBank蛋白编号BC148802
基因名称 RPS6KA6
Gene ID (NCBI) 27330
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UK32
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal s6 kinase4 (RSK4), an X-linked gene, firstly was found as to be a potential tumor suppressive gene in a variety of cancers and is widely participated in signaling pathway. RSK4 is a predominantly cytosolic protein with very low expression and several characteristics of the RSK family kinases, including the presence of two functional kinase domains and a C-terminal docking site for ERK (PMID: 39114716, 30579335). Endogenous RSK4 showed the same level of phosphorylation of Ser232, Ser372, and Ser389 in starved and growth factor-stimulated cells, and mutational analysis demonstrated that these sites are important in stimulation of kinase activity (PMID: 15632195).

实验方案

Product Specific Protocols
IP protocol for RSK4 antibody 21877-1-APDownload protocol
WB protocol for RSK4 antibody 21877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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