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RRP8 Polyclonal antibody

RRP8 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 20129-1-AP

Print datasheet

Synonyms

Cerebral protein 1, KIAA0409, Nucleomethylin, RRP8

验证数据展示

  • WB analysis of human brain using 20129-1-AP

    WB analysis of human brain using 20129-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20129-1-AP (RRP8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 20129-1-AP

    IHC staining of human skin using 20129-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20129-1-AP (RRP8 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 20129-1-AP

    IHC staining of human skin using 20129-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20129-1-AP (RRP8 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 20129-1-AP

    IF Staining of HeLa using 20129-1-AP

    Immunofluorescent analysis of Hela cells, using RRP8 antibody 20129-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20129-1-AP is RRP8 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen RRP8 fusion protein Ag13994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal RNA processing 8, methyltransferase, homolog (yeast)
Synonyms Cerebral protein 1, KIAA0409, Nucleomethylin, RRP8
Calculated Molecular Weight 456 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC001071
Gene Symbol RRP8
Gene ID (NCBI) 23378
RRIDAB_10665422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43159
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RRP8 antibody 20129-1-APDownload protocol
IHC protocol for RRP8 antibody 20129-1-APDownload protocol
IF protocol for RRP8 antibody 20129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols