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验证数据展示

  • WB analysis of mouse thymus using 24490-1-AP

    WB analysis of mouse thymus using 24490-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24490-1-AP (RRP7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 24490-1-AP

    IF Staining of A431 using 24490-1-AP

    Immunofluorescent analysis of A431 cells using 24490-1-AP (RRP7A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human brain using 24490-1-AP

    IHC staining of human brain using 24490-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24490-1-AP (RRP7A Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human placenta using 24490-1-AP

    IHC staining of human placenta using 24490-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24490-1-AP (RRP7A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 24490-1-AP

    IHC staining of human placenta using 24490-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24490-1-AP (RRP7A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RRP7A Polyclonal antibody

RRP7A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24490-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

BK126B4.3, CGI-96, Ribosomal RNA-processing protein 7 homolog A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse thymus tissue
Positive IHC detected inhuman placenta tissue, human brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24490-1-AP targets RRP7A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21636

Product name: Recombinant human RRP7A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-176 aa of BC121118

Sequence: MVARRRKCAARDPEDRIPSPLGYAAIPIKFSEKQQASHYLYVRAHGVRQGTKSTWPQKRTLFVLNVPPYCTEESLSRLLSTCGLVQSVELQEKPDLAESPKESRSKFFHPKPVPGFQVAYVVFQKPSGVSAALALKGPLLVSTESHPVKSGIHKWISDYADSVPDPEALRVEVDTF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal RNA processing 7 homolog A (S. cerevisiae)
别名 BK126B4.3, CGI-96, Ribosomal RNA-processing protein 7 homolog A
计算分子量 280 aa, 32 kDa
观测分子量 42 kDa
GenBank蛋白编号BC121118
基因名称 RRP7A
Gene ID (NCBI) 27341
RRIDAB_2879570
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3A4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RRP7A, also named as Ribosomal RNA-processing protein 7 homolog A, is a 280 amino acid protein, which belongs to the RRP7 family. The calcualted molecular weight of RRP7A is 32 kDa, but modified RRP7A is about 42 kDa.

实验方案

Product Specific Protocols
WB protocol for RRP7A antibody 24490-1-APDownload protocol
IHC protocol for RRP7A antibody 24490-1-APDownload protocol
IF protocol for RRP7A antibody 24490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols