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验证数据展示

  • WB analysis of MCF-7 using 25440-1-AP

    WB analysis of MCF-7 using 25440-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25440-1-AP (RRP36 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 25440-1-AP

    IF Staining of MCF-7 using 25440-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 25440-1-AP (RRP36 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RRP36 Polyclonal antibody

RRP36 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25440-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

C6orf153, HSPC253, RRP36

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25440-1-AP targets RRP36 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RRP36 fusion protein Ag22014 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 6 open reading frame 153
别名 C6orf153, HSPC253, RRP36
计算分子量 259 aa, 30 kDa
观测分子量 30-40 kDa
GenBank蛋白编号BC011933
基因名称 RRP36
Gene ID (NCBI) 88745
RRIDAB_2880082
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EU6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RRP36 antibody 25440-1-APDownload protocol
IF protocol for RRP36 antibody 25440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols