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验证数据展示

  • WB analysis of HEK-293 using 27723-1-AP

    WB analysis of HEK-293 using 27723-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 27723-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27723-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 27723-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27723-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 27723-1-AP

    IF Staining of A431 using 27723-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 27723-1-AP (RRP1B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RRP1B Polyclonal antibody

RRP1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27723-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

RRP1-like protein B, RRP1 like protein B, Ribosomal RNA processing protein 1 homolog B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27723-1-AP targets RRP1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23405

Product name: Recombinant human RRP1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 386-706 aa of BC028386

Sequence: CVEEEDSESSLQKRRRKKKKKHHLQPENPGPGGAAPSLEQNRGREPEASGPKALKARVAEPGAEATSSTGEESGSEHPPAVPMHNKRKRPRKKSPRAHREMLESAVLPPEDMSQSGPSGSHPQGPRGSPTGGAQLLKRKRKLGVVPVNGSGLSTPAWPPLQQEGPPTGPAEGANSHTTLPQRRRLQKKKAGPGSLELCGLPSQKTASLKKRKKMRVMSNLVEHNGVLESEAGQPQALGSSGTCSSLKKQKLRAESDFVKFDTPFLPKPLFFRRAKSSTATHPPGPAVQLNKTPSSSKKVTFGLNRNMTAEFKKTDKSILVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal RNA processing 1 homolog B (S. cerevisiae)
别名 RRP1-like protein B, RRP1 like protein B, Ribosomal RNA processing protein 1 homolog B
观测分子量 84 kDa
GenBank蛋白编号BC028386
基因名称 RRP1B
Gene ID (NCBI) 23076
RRIDAB_2880953
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14684
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal RNA Processing 1 Homolog B (RRP1B) is a protein that has been implicated in several biological processes, particularly in the context of cancer progression and viral replication. RRP1B was first identified as a metastasis susceptibility gene in breast cancer through its ability to modulate gene expression in a manner that can be used to accurately predict prognosis in breast cancer . It has been shown to interact with the metastasis modifier gene, Sipa1, and modulate elements of metastasis predictive gene expression signatures.

实验方案

Product Specific Protocols
WB protocol for RRP1B antibody 27723-1-APDownload protocol
IHC protocol for RRP1B antibody 27723-1-APDownload protocol
IF protocol for RRP1B antibody 27723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols