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验证数据展示

  • WB analysis of NIH/3T3 using 12530-1-AP (same clone as 12530-1-PBS)

    WB analysis of NIH/3T3 using 12530-1-AP (same clone as 12530-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12530-1-AP (RRAS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 12530-1-AP (same clone as 12530-1-PBS)

    IHC staining of mouse heart using 12530-1-AP (same clone as 12530-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RRAS2 antibody included
  • IHC staining of mouse heart using 12530-1-AP (same clone as 12530-1-PBS)

    IHC staining of mouse heart using 12530-1-AP (same clone as 12530-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RRAS2 antibody included
  • IP experiment of NIH/3T3 using 12530-1-AP (same clone as 12530-1-PBS)

    IP experiment of NIH/3T3 using 12530-1-AP (same clone as 12530-1-PBS)

    IP result of anti-RRAS2 (IP:12530-1-AP, 4ug; Detection:12530-1-AP 1:800) with NIH/3T3 cells lysate 4000ug. This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 12530-1-AP (same clone as 12530-1-PBS)

    FC experiment of A431 using 12530-1-AP (same clone as 12530-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RRAS2 (12530-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 12530-1-AP (same clone as 12530-1-PBS)

    IF Staining of A431 using 12530-1-AP (same clone as 12530-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RRAS2 antibody (12530-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

RRAS2 Polyclonal antibody, PBS Only

RRAS2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 12530-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

EC:3.6.5.-, Ras like protein TC21, Ras related protein R Ras2, Ras-like protein TC21, Ras-related protein R-Ras2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RRAS2 Ready-To-Use IHC Kit. RRAS2 primary antibody included.

产品信息

12530-1-PBS targets RRAS2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3226

Product name: Recombinant human RRAS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-204 aa of BC013106

Sequence: MAAAGWRDGSGQEKYRLVVVGGGGVGKSALTIQFIQSYFVTDYDPTIEDSYTKQCVIDDRAARLDILDTAGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMILIGNKADLDHQRQVTQEEGQQLARQLKVTYMEASAKIRMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKKGCHCVIF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 related RAS viral (r-ras) oncogene homolog 2
别名 EC:3.6.5.-, Ras like protein TC21, Ras related protein R Ras2, Ras-like protein TC21, Ras-related protein R-Ras2
计算分子量 204 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC013106
基因名称 RRAS2
Gene ID (NCBI) 22800
RRIDAB_2180206
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62070
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ras-related protein R-Ras2 (RRAS2) is also named TC21 and Teratocarcinoma oncogene. The RRAS2 member of the RAS-related subfamily, also known as TC21, is a close relative of the classic RAS GTPases (PMID: 3898776). RRAS2, a member of the R-Ras subfamily of Ras-like low-molecular-weight GTPases, is located in the plasma membrane and functions as a signal transducer (PMID: 38601074). RRAS2 is GTP-binding protein with GTPase activity, involved in the regulation of MAPK signaling pathway and thereby controlling multiple cellular processes (PMID: 31130282).

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