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验证数据展示

  • WB analysis of Jurkat using 26989-1-AP

    WB analysis of Jurkat using 26989-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 26989-1-AP

    WB analysis of Jurkat using 26989-1-AP

    WB result of RRAGC antibody (26989-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRAGC transfected Jurkat cells.

  • Featured Product
  • KD/KO验证

RRAGC Polyclonal antibody

RRAGC Polyclonal Antibody for WB, ELISA
Cat No. 26989-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA and More (2)

别名

GTPase interacting protein 2, GTR2, Rag C, RAGC, Ras related GTP binding C, RRAGC, TIB929

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells, HEK-293T cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26989-1-AP targets RRAGC in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag25780

Product name: Recombinant human RRAGC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-54 aa of BC016668

Sequence: MSLQYGAEETPLAGSYGAADSFPKDFGYGVEEEEEEAAAAGGGVGAGAGGGCGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras-related GTP binding C
别名 GTPase interacting protein 2, GTR2, Rag C, RAGC, Ras related GTP binding C, RRAGC, TIB929
计算分子量 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC016668
基因名称 RRAGC
Gene ID (NCBI) 64121
RRIDAB_2880714
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HB90
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RRAGC antibody 26989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Autophagy

KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.

Authors - Guokai Yan
View Article
humanWB

Genet Med

De novo missense variants in RRAGC lead to a fatal mTORopathy of early childhood

Authors - Margot R F Reijnders
View Article
human,mouseWB,IHC

Cell Death Dis

Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation

Authors - Mei-Xin Li
View Article
humanWB,IP

Cell Metab

Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism

Authors - Ning Wang
View Article