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验证数据展示

  • WB analysis using 27763-1-AP

    WB analysis using 27763-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27763-1-AP (RRAD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of SKOV-3 using 27763-1-AP

    WB analysis of SKOV-3 using 27763-1-AP

    WB result of RRAD antibody (27763-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRAD transfected SKOV-3 cells.

  • Featured Product
  • KD/KO验证

RRAD Polyclonal antibody

RRAD Polyclonal Antibody for WB, ELISA
Cat No. 27763-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Ras associated with diabetes, RAD1, RAD, GTP-binding protein RAD, GTP binding protein RAD

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, SKOV-3 cells, rat heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27763-1-AP targets RRAD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26869

Product name: Recombinant human RRAD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 39-141 aa of BC057815

Sequence: SMPVDERDLQAALTPGALTAAAAGTGTQGPRLDWPEDSEDSLSSGGSDSDESVYKVLLLGAPGVGKSALARIFGGVEDGPEAEAAGHTYDRSIVVDGEEASLM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras-related associated with diabetes
别名 Ras associated with diabetes, RAD1, RAD, GTP-binding protein RAD, GTP binding protein RAD
计算分子量 33 kDa
观测分子量 29-34 kDa
GenBank蛋白编号BC057815
基因名称 RRAD
Gene ID (NCBI) 6236
RRIDAB_3085993
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55042
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RRAD is also named as RAD1. RRAD, a member of the Ras-like small GTPase family, was initially identified as a gene associated with Type II diabetes since it was found to be overexpressed in some Type II diabetic patients (PMID: 8248782). RRAD overexpression reduced insulin-stimulated glucose uptake in cultured muscle and adipocytes cells (PMID: 8798502). RRAD was found to be frequently down-regulated in different types of human cancers, including lung cancer, breast cancer, and nasopharyngeal carcinoma, etc, due to the hypermethylation of its promoter (PMID: 17195088).

实验方案

Product Specific Protocols
WB protocol for RRAD antibody 27763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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