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  • KD/KO Validated

CNOT9 Polyclonal antibody

CNOT9 Polyclonal Antibody for WB, IP, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF/ICC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 22503-1-AP

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Synonyms

CNOT9, Rcd 1, RCD1, RCD1+, RQCD1



经过测试的应用

Positive WB detected inHeLa cells, mouse thymus tissue, HepG2 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman stomach cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, hTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT9 Ready-To-Use IHC Kit. CNOT9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22503-1-AP targets CNOT9 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CNOT9 fusion protein Ag18197 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RCD1 required for cell differentiation1 homolog (S. pombe)
Synonyms CNOT9, Rcd 1, RCD1, RCD1+, RQCD1
Calculated Molecular Weight 299 aa, 34 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberBC007102
Gene Symbol RQCD1
Gene ID (NCBI) 9125
RRIDAB_11232413
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92600
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RQCD1, also known as CNOT9, is a highly conserved transcriptional cofactor predominantly expressed in actively diffierentiating tissues like testis and thymus. It is a component of the CCR4-NOT complex, the major effector complex in miRNA-mediated gene silencing. Overexpression of RQCD1 has been reported in serveral tumors, indicating that it may be a potential molecular target for cancer treatment.

实验方案

Product Specific Protocols
WB protocol for CNOT9 antibody 22503-1-APDownload protocol
IHC protocol for CNOT9 antibody 22503-1-APDownload protocol
IF protocol for CNOT9 antibody 22503-1-APDownload protocol
IP protocol for CNOT9 antibody 22503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Phase Transitions in the Assembly and Function of Human miRISC.

Authors - Jessica Sheu-Gruttadauria
humanWB

Mol Cell

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

Authors - Markus Höpfler
  • KD Validated
humanWB

Mol Cell

Structural and Biochemical Insights to the Role of the CCR4-NOT Complex and DDX6 ATPase in MicroRNA Repression.

Authors - Hansruedi Mathys
humanWB

Mol Cell

A DDX6-CNOT1 Complex and W-Binding Pockets in CNOT9 Reveal Direct Links between miRNA Target Recognition and Silencing.

Authors - Ying Chen
humanWB,IP

Nat Commun

RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation.

Authors - Fabian Poetz
  • KD Validated
humanWB

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic