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验证数据展示

  • WB analysis of mouse uterus using 18215-1-AP

    WB analysis of mouse uterus using 18215-1-AP

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 18215-1-AP

    IHC staining of human lung using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPS9 antibody included
  • IHC staining of human lung using 18215-1-AP

    IHC staining of human lung using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPS9 antibody included
  • IHC staining of mouse lung using 18215-1-AP

    IHC staining of mouse lung using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPS9 antibody included
  • IHC staining of mouse pancreas using 18215-1-AP

    IHC staining of mouse pancreas using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPS9 antibody included
  • IHC staining of mouse stomach using 18215-1-AP

    IHC staining of mouse stomach using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPS9 antibody included
  • IF Staining of HeLa using 18215-1-AP

    IF Staining of HeLa using 18215-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPS9 antibody (18215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

RPS9 Polyclonal antibody

RPS9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18215-1-AP

产品说明书

引用文献(10)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

40S ribosomal protein S9, ribosomal protein S9, Small ribosomal subunit protein uS4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse uterus tissue, HeLa cells, mouse ovary tissue
Positive IHC detected inhuman lung tissue, human ovary tissue, human skin tissue, human testis tissue, mouse lung tissue, mouse pancreas tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPS9 Ready-To-Use IHC Kit. RPS9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18215-1-AP targets RPS9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag12945

Product name: Recombinant human RPS9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-194 aa of BC000802

Sequence: MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRRLVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S9
别名 40S ribosomal protein S9, ribosomal protein S9, Small ribosomal subunit protein uS4
计算分子量 23 kDa
观测分子量 25 kDa
GenBank蛋白编号BC000802
基因名称 RPS9
Gene ID (NCBI) 6203
RRIDAB_2182390
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46781
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPS9 antibody 18215-1-APDownload protocol
IHC protocol for RPS9 antibody 18215-1-APDownload protocol
IF protocol for RPS9 antibody 18215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Dev Cell

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Authors - Yuqing Lei
View Article
humanWB

Proc Natl Acad Sci U S A

Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.

Authors - Elizabeth I Vink
  • KD Validated
View Article
humanIHC

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis
View Article
humanWB

Sci Rep

Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway.

Authors - Haiping Tang
View Article
humanWB

Front Immunol

Gradient Boosting Machine Learning Model for Defective Endometrial Receptivity Prediction by Macrophage-Endometrium Interaction Modules.

Authors - Bohan Li
View Article
humanWB

Genes (Basel)

Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.

Authors - Meijuan Zou
  • KD Validated
View Article