验证数据展示
经过测试的应用
| Positive WB detected in | mouse uterus tissue, HeLa cells, mouse ovary tissue |
| Positive IHC detected in | human lung tissue, human ovary tissue, human skin tissue, human testis tissue, mouse lung tissue, mouse pancreas tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy RPS9 Ready-To-Use IHC Kit. RPS9 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18215-1-AP targets RPS9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12945 Product name: Recombinant human RPS9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-194 aa of BC000802 Sequence: MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRRLVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S9 |
| 别名 | 40S ribosomal protein S9, ribosomal protein S9, Small ribosomal subunit protein uS4 |
| 计算分子量 | 23 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC000802 |
| 基因名称 | RPS9 |
| Gene ID (NCBI) | 6203 |
| RRID | AB_2182390 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P46781 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS9 antibody 18215-1-AP | Download protocol |
| IHC protocol for RPS9 antibody 18215-1-AP | Download protocol |
| WB protocol for RPS9 antibody 18215-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Psychiatry Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome | ||
J Transl Med Paternal ribosome biogenesis dysfunction caused by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice. | ||
Proc Natl Acad Sci U S A Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.
| ||
Dev Cell Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility. | ||
