验证数据展示
经过测试的应用
| Positive WB detected in | HuH-7 cells, HT-29 cells, LoVo cells, HCT 116 cells, HepG2 cells, NIH/3T3 cells, rat liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87092-1-RR targets RPS7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5999 Product name: Recombinant human RPS7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-194 aa of BC061901 Sequence: MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLVRELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDKAQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S7 |
| 别名 | 40S ribosomal protein S7, DBA8, ribosomal protein S7, RPS 7, Small ribosomal subunit protein eS7 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22-25 kDa |
| GenBank蛋白编号 | BC061901 |
| 基因名称 | RPS7 |
| Gene ID (NCBI) | 6201 |
| RRID | AB_3745331 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62081 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomal protein S7(RPS7) belongs to the RPs, which is structural compoent of the ribosome invilved in protein synthesis. It interacts with and inhibit mdm2 mediated p53 degradation function. Also RPS7 has a essential role in early development. RPS7 involves in the maturation of ribosomal RNAs in the large or the small ribosomal subunit production pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS7 antibody 87092-1-RR | Download protocol |
| WB protocol for RPS7 antibody 87092-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
